DOST-PCAARRD, PCA continue studies on coconut embryogenesis

Field-planted plumule-derived seedlings produced through coconut somatic embryogenesis technology (CSet) at the PCA-ZRC. (Image credit: PCA-ZRC)

The improvement of coconut somatic embryogenesis technology (CSet) gets another year of funding support from the Philippine Council for Agriculture, Aquatic and Natural Resources Research and Development of the Department of Science and Technology (DOST-PCAARRD).

CSet is a propagation technique of coconut planting materials using plumular tissues of identified tall, dwarf, and hybrid varieties. It aims to increase the production of quality planting materials of selected coconut varieties in the country.

The projects, “Propagation of Quality Planting Materials of Baybay Tall (BAYT), Laguna Tall (LAGT), and Tacunan Dwarf (TACD) Coconut Varieties through CSet” and “Propagation of Quality Planting Materials of Selected Tall, Dwarf, and Hybrid Coconut Varieties through CSet,” are being led by Dr. Susan M. Rivera of the Philippine Coconut Authority-Zamboanga Research Center (PCA-ZRC) and Dr. Cristeta A. Cueto of the PCA-Albay Research Center (PCA-ARC), respectively.

Ex vitro established plantlets of BAYT produced through CSet, ready for field planting (Image Credit: PCA-ARC)

Both projects maintain and produce quality planting materials of selected coconut varieties, while also exploring protocol improvement to increase the regeneration efficiency of the PCA-ARC CSet protocol. Using the secondary somatic embryogenesis pathway, the number of somatic embryos and regenerants produced is expected to hasten and increase.

Likewise, modifications to the ex vitro transfer protocol are underway to increase the survival rate of both fully developed and immature plantlets. Ex vitro pertains to growing the coconut planting material outside the tissue culture or in soil or potting mixture. Several CSet-derived planting materials were already planted in the PCA-ZRC compound in Zamboanga City early this year, while those from PCA-ARC will be planted by January 2022.

The research teams from PhilRice, PCA-ZRC, PCA-ARC with the S&T Consultants and DOST-PCAARRD representatives during the Review and Evaluation of DOST-PCAARRD-funded projects on rice and coconut held on September 29, 2021, via Video Conferencing. (Image Crops Research Division, DOST-PCAARRD)

Both projects maintain and produce quality planting materials of selected coconut varieties, while also exploring protocol improvement to increase the regeneration efficiency of the PCA-ARC CSet protocol. Using the secondary somatic embryogenesis pathway, the number of somatic embryos and regenerants produced is expected to hasten and increase.

Likewise, modifications to the ex vitro transfer protocol are underway to increase the survival rate of both fully developed and immature plantlets. Ex vitro pertains to growing the coconut planting material outside the tissue culture or in soil or potting mixture. Several CSet-derived planting materials were already planted in the PCA-ZRC compound in Zamboanga City early this year, while those from PCA-ARC will be planted by January 2022. (Alissa Carol M. Ibarra/DOST-PCAARRD S&T Media Services)